Chromosomal effects of adeno-associated virus vector integration

被引：123

作者：

Miller, DG ^{[1
]}

Rutledge, EA ^{[1
]}

Russell, DW ^{[1
]}

机构：

[1] Univ Washington, Dept Med, Seattle, WA 98195 USA

来源：

NATURE GENETICS | 2002年 / 30卷 / 02期

关键词：

D O I：

10.1038/ng824

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Adeno-associated virus (AAV) vectors are currently being used in several clinical gene-therapy trials (see the NIH OBA Human Gene Transfer Clinical Trials Database); however, little is known about the chromosomal effects of vector integration. Here we report that integrated vector proviruses are associated with chromosomal deletions and other rearrangements and are frequently located on chromosome 19 (although not at the wildtype AAV integration site).

引用

页码：147 / 148

页数：2

共 10 条

[1] DNA-DAMAGING AGENTS GREATLY INCREASE THE TRANSDUCTION OF NONDIVIDING CELLS BY ADENOASSOCIATED VIRUS VECTORS
ALEXANDER, IE
RUSSELL, DW
MILLER, AD
[J]. JOURNAL OF VIROLOGY, 1994, 68 (12) : 8282 - 8287
[2] RECOMBINANT JUNCTIONS FORMED BY SITE-SPECIFIC INTEGRATION OF ADENOASSOCIATED VIRUS INTO AN EPISOME
GIRAUD, C
WINOCOUR, E
BERNS, KI
[J]. JOURNAL OF VIROLOGY, 1995, 69 (11) : 6917 - 6924
[3] Design and packaging of adeno-associated virus gene targeting vectors
Hirata, RK
Russell, DW
[J]. JOURNAL OF VIROLOGY, 2000, 74 (10) : 4612 - 4620
[4] CHARACTERIZATION OF A PREFERRED SITE ON HUMAN CHROMOSOME-19Q FOR INTEGRATION OF ADENOASSOCIATED VIRUS-DNA BY NONHOMOLOGOUS RECOMBINATION
KOTIN, RM
LINDEN, RM
BERNS, KI
[J]. EMBO JOURNAL, 1992, 11 (13) : 5071 - 5078
[5] Site-specific integration by adeno-associated virus
Linden, RM
Ward, P
Giraud, C
Winocour, E
Berns, KI
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (21) : 11288 - 11294
[6] Isolation of recombinant adeno-associated virus vector-cellular DNA junctions from mouse liver
Nakai, H
Iwaki, Y
Kay, MA
Couto, LB
[J]. JOURNAL OF VIROLOGY, 1999, 73 (07) : 5438 - 5447
[7] DNA-SYNTHESIS AND TOPOISOMERASE INHIBITORS INCREASE TRANSDUCTION BY ADENOASSOCIATED VIRUS VECTORS
RUSSELL, DW
ALEXANDER, IE
MILLER, AD
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (12) : 5719 - 5723
[8] Adeno-associated virus vector integration junctions
Rutledge, EA
Russell, DW
[J]. JOURNAL OF VIROLOGY, 1997, 71 (11) : 8429 - 8436
[9] Cellular recombination pathways and viral terminal repeat hairpin structures are sufficient for adeno-associated virus integration in vivo and in vitro
Yang, CC
Xiao, X
Zhu, X
Ansardi, DC
Epstein, ND
Frey, MR
Matera, AG
Samulski, RJ
[J]. JOURNAL OF VIROLOGY, 1997, 71 (12) : 9231 - 9247
[10] Roles of adeno-associated virus Rep protein and human chromosome 19 in site-specific recombination
Young, SM
McCarty, DM
Degtyareva, N
Samulski, RJ
[J]. JOURNAL OF VIROLOGY, 2000, 74 (09) : 3953 - 3966

← 1 →