Proteome analysis of soybean hypocotyl and root under salt stress

被引:130
作者
Aghaei, K. [1 ,2 ]
Ehsanpour, A. A. [2 ]
Shah, A. H. [1 ]
Komatsu, S. [1 ]
机构
[1] Natl Inst Crop Sci, Tsukuba, Ibaraki 3058518, Japan
[2] Isfahan Univ, Fac Sci, Dept Biol, Esfahan, Iran
关键词
Proteome; Soybean; Salt stress; Proline; RESOLUTION 2-DIMENSIONAL ELECTROPHORESIS; EMBRYOGENESIS-ABUNDANT PROTEIN; PROLINE ACCUMULATION; GEL-ELECTROPHORESIS; MESSENGER-RNA; ABSCISIC-ACID; WATER-STRESS; GLYCINE-MAX; TOLERANCE; RICE;
D O I
10.1007/s00726-008-0036-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To evaluate the response of soybean to salt stress, the related changes in protein expression were investigated using the proteomic approach. Soybean plants were exposed to 0, 50, 100, and 200 mM NaCl. Especially at 200 mM, the length and fresh weight of the hypocotyl and root reduced under salt stress, while the proline content increased. Proteins from the hypocotyl and root treated with 100 mM NaCl were extracted and separated by two-dimensional polyacrylamide gel electrophoresis; 321 protein spots were detected. In response to salt stress, seven proteins were reproducibly found to be up- or down-regulated by two to sevenfold: late embryogenesis-abundant protein, beta-conglycinin, elicitor peptide three precursor, and basic/helix-loop-helix protein were up-regulated, while protease inhibitor, lectin, and stem 31-kDa glycoprotein precursor were down-regulated. These results indicate that salinity can change the expression level of some special proteins in the hypocotyl and root of soybean that may in turn play a role in the adaptation to saline conditions.
引用
收藏
页码:91 / 98
页数:8
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