A new iridovirus isolated from soft-shelled turtle

被引：81

作者：

Chen, ZX ^{[1
]}

Zheng, JC ^{[1
]}

Jiang, YL ^{[1
]}

机构：

[1] Shenzhen Anim & Plant Quarantine Bur, Shenzhen 518010, Peoples R China

来源：

VIRUS RESEARCH | 1999年 / 63卷 / 1-2期

关键词：

iridovirus; red neck disease; soft-shelled turtle (Trionyx sinensis);

D O I：

10.1016/S0168-1702(99)00069-6

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A virus was isolated from soft-shelled turtle (Trionyx sinensis) with 'red neck disease' on a farm in Shenzhen, China. the virus multiplied and caused a cytopathogenic effect (CPE) at 15-30 degrees C in CO, FHM, CK and BF-2 cells. The optimum conditions for replication was in CO cells at 25-30 degrees C. The virus was sensitive to chloroform treatment, acid (pH 3) or alkaline (pH 10) conditions and heating at 56 degrees C for 30 min. Treatment with 5-iodo-2-deoxyuridine (IUDR) inhibited viral replication indicating the presence of a DNA. genome. Electron microscopy of infected CO culture fluid revealed spherical particles measuring 120-160 nm in diameter. Observation of ultra-thin sections showed numerous hexagonal viral particles in the cytoplasm and nucleus of cells typical of an iridovirus. This virus was moderately virulent for turtles in infection tests. We suggest that this virus is named soft-shelled turtle iridovirus (STIV). (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：147 / 151

页数：5

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