Identification of false-positive CBFβ/MYH11 RT-PCR results

被引：12

作者：

Hackwell, SM ^{[1
]}

Robinson, DO ^{[1
]}

Harvey, JF ^{[1
]}

Ross, FM ^{[1
]}

机构：

[1] Salisbury Dist Hosp, Wessex Reg Genet Lab, Salisbury SP2 8BJ, Wilts, England

来源：

LEUKEMIA | 1999年 / 13卷 / 10期

关键词：

acute myeloid leukaemia; CBF beta/MYH11 transcripts; RT-PCR;

D O I：

10.1038/sj.leu.2401545

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Persistent problems with false positive results were encountered when carrying out a published RT-PCR method to detect the CBF beta/MYH11 transcripts associated with the inv(16)(p13q22) cytogenetic abnormality in acute myeloid leukaemia. These were shown to be due to amplification of part of the intronic MYH11 sequence, presumably from very small amounts of contaminating DNA or unspliced primary RNA transcripts, amplified because of partial homology of the CBF beta 3 primer to intronic MYH11 sequence.

引用

页码：1617 / 1619

页数：3

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