SecE-depleted membranes of Escherichia coli are active - SecE is not obligatorily required for the in vitro translocation of certain protein precursors

被引:32
作者
Yang, YB [1 ]
Yu, NJ [1 ]
Tai, PC [1 ]
机构
[1] GEORGIA STATE UNIV, DEPT BIOL, ATLANTA, GA 30303 USA
关键词
D O I
10.1074/jbc.272.21.13660
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Membrane vesicles were prepared from Escherichia coli cells in which SecE was depleted to 2% of wild-type membranes. SecE depletion had pleiotropic effects; SecD, SecF, SecG, and SecY were decreased 4-6-fold, whereas SecA was increased about 16-fold over that of wild-type membranes. These membranes were substantially active in the in vitro translocation of proOmpA, which was mediated by the SecA pathway since it was inhibited by azide. Similar substantial translocation activities were observed for proLamB and proLpp in the SecE depleted membranes. However, the translocation of proPhoA was more severely impaired. These data indicate that SecE may enhance but is not obligatorily required for the translocation of at least certain precursors, and suggest that the effects of the SecE depletion on protein translocation may be precursor-dependent.
引用
收藏
页码:13660 / 13665
页数:6
相关论文
共 56 条
[1]   RECONSTITUTION OF A PROTEIN TRANSLOCATION SYSTEM CONTAINING PURIFIED SECY, SECE, AND SECA FROM ESCHERICHIA-COLI [J].
AKIMARU, J ;
MATSUYAMA, SI ;
TOKUDA, H ;
MIZUSHIMA, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (15) :6545-6549
[2]   SECD AND SECF ARE REQUIRED FOR THE PROTON ELECTROCHEMICAL GRADIENT STIMULATION OF PREPROTEIN TRANSLOCATION [J].
ARKOWITZ, RA ;
WICKNER, W .
EMBO JOURNAL, 1994, 13 (04) :954-963
[3]   PRLA (SECY) AND PRLG (SECE) INTERACT DIRECTLY AND FUNCTION SEQUENTIALLY DURING PROTEIN TRANSLOCATION IN ESCHERICHIA-COLI [J].
BIEKER, KL ;
SILHAVY, TJ .
CELL, 1990, 61 (05) :833-842
[4]   SUPPRESSOR ANALYSIS SUGGESTS A MULTISTEP, CYCLIC MECHANISM FOR PROTEIN SECRETION IN ESCHERICHIA-COLI [J].
BIEKERBRADY, K ;
SILHAVY, TJ .
EMBO JOURNAL, 1992, 11 (09) :3165-3174
[5]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[6]   PHAGE SHOCK PROTEIN, A STRESS PROTEIN OF ESCHERICHIA-COLI [J].
BRISSETTE, JL ;
RUSSEL, M ;
WEINER, L ;
MODEL, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (03) :862-866
[7]   THE PURIFIED ESCHERICHIA-COLI INTEGRAL MEMBRANE-PROTEIN SECY/E IS SUFFICIENT FOR RECONSTITUTION OF SECA-DEPENDENT PRECURSOR PROTEIN TRANSLOCATION [J].
BRUNDAGE, L ;
HENDRICK, JP ;
SCHIEBEL, E ;
DRIESSEN, AJM ;
WICKNER, W .
CELL, 1990, 62 (04) :649-657
[8]  
BRUNDAGE L, 1992, J BIOL CHEM, V267, P4166
[9]   Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii [J].
Bult, CJ ;
White, O ;
Olsen, GJ ;
Zhou, LX ;
Fleischmann, RD ;
Sutton, GG ;
Blake, JA ;
FitzGerald, LM ;
Clayton, RA ;
Gocayne, JD ;
Kerlavage, AR ;
Dougherty, BA ;
Tomb, JF ;
Adams, MD ;
Reich, CI ;
Overbeek, R ;
Kirkness, EF ;
Weinstock, KG ;
Merrick, JM ;
Glodek, A ;
Scott, JL ;
Geoghagen, NSM ;
Weidman, JF ;
Fuhrmann, JL ;
Nguyen, D ;
Utterback, TR ;
Kelley, JM ;
Peterson, JD ;
Sadow, PW ;
Hanna, MC ;
Cotton, MD ;
Roberts, KM ;
Hurst, MA ;
Kaine, BP ;
Borodovsky, M ;
Klenk, HP ;
Fraser, CM ;
Smith, HO ;
Woese, CR ;
Venter, JC .
SCIENCE, 1996, 273 (5278) :1058-1073
[10]  
CABELLI RJ, 1991, J BIOL CHEM, V266, P24420