Genomic variation of Bartonella henselae strains detected in lymph nodes of patients with cat scratch disease

被引:81
作者
Zeaiter, Z [1 ]
Fournier, PE [1 ]
Raoult, D [1 ]
机构
[1] Fac Med Marseille, CNRS UPRES A 6020, Unite Rickettsies, F-13385 Marseille 05, France
关键词
D O I
10.1128/JCM.40.3.1023-1030.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Bartonella henselae is the primary agent of cat scratch disease (CSD). In order to study the: genetic variation of B. henselae and the correlation of the various genotypes with epidemiological and clinical findings, two seminested, groEL- and pap31-based PCR assays were carried out with specimens from 273 patients. Amplicons were sequenced to determine the genotype of the causative Bartonella species. Compared to our reference intergenic spacer region-based PCR, the groEL- and pap31-based assays were 1.7 and 1.9 times more sensitive, respectively. All 107 positive patients were infected with B. henselae; neither Bartonella clarridgeiae nor other species were detected. Based on the groEL and pap31 sequences, B. henselae amplicons were classified into two genogroups, Marseille and Houston-1, and into four variants, Marseille, CAL-1, Houston-1, and a new variant, ZF-1. Patients infected with either one or the other genogroup did not exhibit different epidemiological or clinical characteristics. Our study highlights the genotypic heterogeneity of B. henselae in patients with CSD.
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页码:1023 / 1030
页数:8
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