Reversible Accumulation of PEGylated Single-Walled Carbon Nanotubes in the Mammalian Nucleus

被引:120
作者
Cheng, Jinping [1 ]
Fernando, K. A. Shiral [2 ,3 ]
Veca, L. Monica [2 ,3 ]
Sun, Ya-Ping [2 ,3 ]
Lamond, Angus I. [4 ]
Lam, Yun Wah [1 ]
Cheng, Shuk Han [1 ]
机构
[1] City Univ Hong Kong, Dept Biol & Chem, Hong Kong, Hong Kong, Peoples R China
[2] Clemson Univ, Dept Chem, Clemson, SC 29634 USA
[3] Clemson Univ, Lab Emerging Mat & Technol, Clemson, SC 29634 USA
[4] Univ Dundee, Coll Life Sci, Wellcome Trust Ctr Gene Regulat & Express, Dundee DD1 5EH, Scotland
基金
美国国家科学基金会;
关键词
carbon nanotubes; nucleus; live-cell imaging; fluorescence recovery after photobleaching; cell penetration; cellular efflux;
D O I
10.1021/nn800461u
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Carbon nanotubes (CNTs) have been shown to cross cell membranes and can mediate the internalization of macromolecules. These characteristics have constituted CNTs as an exciting new tool for drug delivery and biological sensing. While CNTs exhibit great potential in biomedical and pharmaceutical applications, neither the cell penetration mechanism of CNTs nor the intracellular fate of the internalized CNTs are fully understood. In this study, time-lapse fluorescence microscopy was used to investigate the intracellular distribution of FITC labeled PEGylated single-walled CNTs (FITC-PEG-SWCNTs) in living cells and shown that PEGylated SWCNTs entered the nucleus of several mammalian cell lines in an energy-dependent process. The presence of FITC-PEG-SWCNTs in the cell nucleus did not cause discernible changes in the nuclear organization and had no effect on the growth kinetics and cell cycle distribution for up to 5 days. Remarkably, upon removal of the FITC-PEG-SWCNTS from the culture medium, the internalized FITC-PEG-SWCNTs rapidly moved out of the nucleus and were released from the cells. Thus, the intracellular PEGylated SWCNTs were highly dynamic and the cell penetration of PEGylated SWCNTs appeared as bidirectional. These observations suggest SWCNTs may be used as an ideal nanovector in biomedical and pharmaceutical applications.
引用
收藏
页码:2085 / 2094
页数:10
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