Development of EST-SSRs in Finger Millet (Eleusine coracana ssp coracana) and their Transferability to Pearl Millet (Pennisetum glaucum)

被引：20

作者：

Arya, Lalit ^{[1
]}

Verma, Manjusha ^{[1
]}

Gupta, V. K. ^{[1
]}

Karihaloo, J. L. ^{[1
]}

机构：

[1] Natl Bur Plant Genet Resources, Natl Res Ctr DNA Fingerprinting, New Delhi 110012, India

来源：

JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY | 2009年 / 18卷 / 01期

关键词：

ESTs; SSRs; alleles; finger millet; pearl millet; MARKERS; BARLEY; DNA; L;

D O I：

10.1007/BF03263303

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

EST-SSR markers were developed using sequence information from 1740 expressed sequence tags (ESTs) of finger millet available in the public domain. A set of 31 SSR markers were synthesized based on di, tri, tetra and penta-nucleotide repeat sequences. These were used for PCR analysis of 11 elite germplasm lines of finger millet of Indian and African origin. Out of 31 SSR markers, amplification products were obtained for 17 primer pairs. Of these nine were found polymorphic with two alleles per locus. These 17 SSR primer pairs were also tested for amplification in three varieties of pearl millet (Pennisetum glaucum) and 11 could be transferred to pearl millet. The informative EST-SSR markers developed, can be used in finger millet as well as pearl millet genetic improvement projects.

引用

页码：97 / 100

页数：4

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