Protection of mice against brucellosis by vaccination with Brucella melitensis WR201(16MΔpurEK)

被引:39
作者
Hoover, DL [1 ]
Crawford, RM
Van de Verg, LL
Izadjoo, MJ
Bhattacharjee, AK
Paranavitana, CM
Warren, RL
Nikolich, MP
Hadfield, TL
机构
[1] Walter Reed Army Med Ctr, Walter Reed Army Inst Res, Dept Bacterial Dis, Washington, DC 20307 USA
[2] Armed Forces Inst Pathol, Amer Registry Pathol, Washington, DC 20306 USA
[3] Armed Forces Inst Pathol, Dept Infect & Parasit Dis, Washington, DC 20306 USA
关键词
D O I
10.1128/IAI.67.11.5877-5884.1999
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Human brucellosis can be acquired from infected animal tissues by ingestion, inhalation, or contamination of the conjunctiva or traumatized skin by infected animal products. A vaccine to protect humans from occupational exposure or from zoonotic infection in areas where the disease is endemic would reduce an important cause of morbidity worldwide. Vaccines currently used in animals are unsuitable for human use. We tested a live, attenuated, purine-auxotrophic mutant strain of Brucella melitensis, WR201, for its ability to elicit cellular and humoral immune responses and to protect mice against intranasal challenge with B. melitensis 16M. Mice inoculated intraperitoneally with WR201 made serum antibody to lipopolysaccharide and non-O-polysaccharide antigens. Splenocytes from immunized animals released interleukin-2 (IL-2), gamma interferon, and IL-10 when cultured with Brucella antigens. Immunization led to protection from disseminated infection but had only a slight effect on clearance of the challenge inoculum from the lungs. These studies suggest that WR201 should be further investigated as a vaccine to prevent human brucellosis.
引用
收藏
页码:5877 / 5884
页数:8
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