Enhanced mitotic division of cultured Passiflora and Petunia protoplasts by oxygenated perfluorocarbon and haemoglobin

被引：19

作者：

Anthony, P

Davey, MR

Power, JB

Lowe, KC

机构：

[1] Department of Life Science, University of Nottingham

来源：

BIOTECHNOLOGY TECHNIQUES | 1997年 / 11卷 / 08期

关键词：

D O I：

10.1023/A:1018447007628

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cell suspension-derived protoplasts were cultured in (A) liquid medium, (B) medium overlaying oxygenated perfluorodecalin (PFC), (C) medium containing 1:50 (v:v) of haemoglobin solution (Erythrogen(TM)), or (D) medium with 1:50 (v:v) Erythrogen(TM) overlaying oxygenated PFC. In Passiflora, mitotic division of protoplasts was increased (P < 0.05) by all treatments, with Erythrogen(TM) being the most effective (120% increase over control). For Petunia, treatment D induced maximum mitosis (140% over control), whilst Erythrogen(TM) alone produced a less pronounced (80% over control) increase.

引用

页码：581 / 584

页数：4

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