In vitro hydration kinetics of recent post-mortem tissue versus pre-dried corneal stromal tissue

Both recent post-mortem and pre-dried corneal tissue has been used for laboratory studies of stromal swelling, but it has yet to be defined whether the same hydration (H value, mg H2O/mg dry mass) is obtained after extended re-hydration. Fresh or pre-dried pieces (8 x 8 mm squares) of ovine stromas were immersed in 1% NaCl for 24 hr at 37 degrees C, with the wet mass assessed regularly. Pre-drying was achieved in air for 7 days (with sulphuric acid, CaSO4 or silica gel as desiccants), or in an oven for 24 hr at 60, 70 or 80 degrees C. Fresh stroma preparations (less than or equal to 2 hr post-mortem) had initial H values of 3.1, which increased to 27.8 after 24 hr in saline. After pre-drying over sulphuric acid, CaSO, or silica gel, the H values after 24 hr in saline were 19.1, 13.1 and 7.3 respectively (all statistically different from fresh tissue (P < 0.01). Following oven drying at 60, 70, and 80 degrees C, final H values of 8.5, 6.6, and 5.0 were obtained after 24 hr in saline. Recent post-mortem material showed sustained swelling over at least 24 hr. For pre-dried tissue, initial rates of hydration change over 1 hr were higher, but subsequent rates were substantially less after 2 hr. These studies indicate that fresh tissue should be used in studies concerning the swelling properties of the corneal stroma. (C) 1997 Academic Press Limited.