A twinned monoclinic crystal form of human peroxiredoxin 5 with eight molecules in the asymmetric unit

被引:31
作者
Declercq, JP [1 ]
Evrard, C [1 ]
机构
[1] Catholic Univ Louvain, Unit Struct Chem, CSTR, B-1348 Louvain, Belgium
来源
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2001年 / 57卷
关键词
D O I
10.1107/S0907444901015475
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The monoclinic crystal form of human peroxiredoxin 5 with eight molecules in the asymmetric unit was obtained under exactly the same conditions as the tetragonal form with one molecule in the asymmetric unit, except that the latter was briefly cryosoaked with halide for derivatization. A merohedral twinning was observed, which is rather unusual in the monoclinic system and only possible with particular unit-cell dimensions. After detwinning the native and a mercury derivative, the structure was solved by the SIR method with the help of the non-crystallographic symmetry. The packing of the monoclinic and tetragonal forms are compared, with special attention to the role of bromide ions in the change of space group after crystallization. The availability of nine (eight monoclinic plus one tetragonal) independent molecules allows an analysis of the mobility. The two Cys residues implicated in the peroxide-reduction mechanism are located in rigid regions but are covered by mobile loops.
引用
收藏
页码:1829 / 1835
页数:7
相关论文
共 31 条
[1]   THE CCP4 SUITE - PROGRAMS FOR PROTEIN CRYSTALLOGRAPHY [J].
BAILEY, S .
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 :760-763
[2]   FREE R-VALUE - A NOVEL STATISTICAL QUANTITY FOR ASSESSING THE ACCURACY OF CRYSTAL-STRUCTURES [J].
BRUNGER, AT .
NATURE, 1992, 355 (6359) :472-475
[3]   CLONING AND SEQUENCING OF THIOL-SPECIFIC ANTIOXIDANT FROM MAMMALIAN BRAIN - ALKYL HYDROPEROXIDE REDUCTASE AND THIOL-SPECIFIC ANTIOXIDANT DEFINE A LARGE FAMILY OF ANTIOXIDANT ENZYMES [J].
CHAE, HZ ;
ROBISON, K ;
POOLE, LB ;
CHURCH, G ;
STORZ, G ;
RHEE, SG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (15) :7017-7021
[4]  
CHAE HZ, 1994, J BIOL CHEM, V269, P27670
[5]   Crystal structure of a novel human peroxidase enzyme at 2.0 Å resolution [J].
Choi, HJ ;
Kang, SW ;
Yang, CH ;
Rhee, SG ;
Ryu, SE .
NATURE STRUCTURAL BIOLOGY, 1998, 5 (05) :400-406
[6]  
COWTAN K, 1994, JOINT CCP4 ESF EACBM, V31, P24
[7]   Novel approach to phasing proteins: derivatization by short cryo-soaking with halides [J].
Dauter, Z ;
Dauter, M ;
Rajashankar, KR .
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2000, 56 :232-237
[8]   Practical experience with the use of halides for phasing macromolecular structures: a powerful tool for structural genomics [J].
Dauter, Z ;
Li, M ;
Wlodawer, A .
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2001, 57 :239-249
[9]   Entering a new phase: Using solvent halide ions in protein structure determination [J].
Dauter, Z ;
Dauter, M .
STRUCTURE, 2001, 9 (02) :R21-R26
[10]   Crystal structure of human peroxiredoxin 5, a novel type of mammalian peroxiredoxin at 1.5 Å resolution [J].
Declercq, JP ;
Evrard, C ;
Clippe, A ;
Vander Stricht, D ;
Bernard, A ;
Knoops, B .
JOURNAL OF MOLECULAR BIOLOGY, 2001, 311 (04) :751-759