Determination of the xenoestrogens 4-nonylphenol and bisphenol A by high-performance liquid chromatography and fluorescence detection after derivatisation with dansyl chloride

被引：42

作者：

Naassner, M

Mergler, M

Wolf, K

Schuphan, I

机构：

[1] Rhein Westfal TH Aachen, Inst Biol Mikrobiol 4, D-52056 Aachen, Germany

[2] Rhein Westfal TH Aachen, Inst Biol Okol Okotoxikol Okochem 5, D-52056 Aachen, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 2002年 / 945卷 / 1-2期

关键词：

nonylphenols; phenols; bisphenol A; xenoestrogens;

D O I：

10.1016/S0021-9673(01)01522-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An easily performable and highly selective method for the determination of the xenoestrogens bisphenol A (BPA) and technical 4-nonylphenol (mixture of isomers) from environmental samples was developed. The method consists of fluorigenic labelling of the substances by dansylation followed by HPLC separation of the derivatives. Specific wavelengths (lambda(ex)=354 nm, lambda(em)=545 nm) for detection of the dansylated phenols were determined in order to reduce the signals of interfering compounds. The applicablility of the method for environmental samples was demonstrated by using sewage sludge spiked with BPA and 4-n-nonylphenol (as internal standard). (C) 2002 Elsevier Science B.V. All rights reserved.

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页码：133 / 138

页数：6

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