Gas chromatography/mass spectrometry in metabolic profiling of biological fluids

被引：232

作者：

Pasikanti, Kishore K. ^{[1
]}

Ho, P. C. ^{[1
]}

Chan, E. C. Y. ^{[1
]}

机构：

[1] Natl Univ Singapore, Fac Sci, Dept Pharm, Singapore 117543, Singapore

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2008年 / 871卷 / 02期

关键词：

metabolic profiling; gas chromatography; GC/MS; metabonomics; metabolomics; urine; blood;

D O I：

10.1016/j.jchromb.2008.04.033

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

One of the objectives of rnetabonomics is to identify subtle changes in metabolite profiles between biological systems of different physiological or pathological states. Gas chromatography mass spectrometry (GC/MS) is a widely used analytical tool for metabolic profiling in various biofluids, Such as urine and blood due to its high sensitivity, peak resolution and reproducibility. The availability of the GC/MS electron impact (EI) spectral library further facilitates the identification of diagnostic biomarkers and aids the subsequent mechanistic elucidation of the biological or pathological variations. With the advent of new comprehensive two dimensional GC (CC x GC) coupled to time-of-flight mass spectrometry (TOFMS), it is possible to detect more than 1200 compounds in a single analytical run. In this review, we discuss the applications of GC/MS in the metabolic profiling of urine and blood, and discuss its advances in methodologies and technologies. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：202 / 211

页数：10

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