Molecular cloning, expression and characterization of the Canis familiaris interleukin-4

被引：26

作者：

Wondimu, A

Veit, M

Kohn, B

Kaul, S

Hoffmann, A

Brunnberg, L

Schmidt, MFG

机构：

[1] Free Univ Berlin, Inst Immunol & Mol Biol, D-10115 Berlin, Germany

[2] Free Univ Berlin, Clin Small Anim, D-10115 Berlin, Germany

[3] Paul Ehrlich Inst, D-63225 Langen, Germany

来源：

CYTOKINE | 2001年 / 16卷 / 03期

关键词：

interleukin; 4; canine cytokines; recombinant cytokines; expression vectors;

D O I：

10.1006/cyto.2001.0953

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Interleukin 4 (IL-4) is an important regulatory cytokine produced by activated T lymphocytes and mast cells, and regulates the growth and differentiation of cells such as B and T lymphocytes. The rapid amplification of cDNA ends (RACE) was used to clone the canine IL-4 gene. It was expressed in mammalian cells and Escherichia coli. Monoclonal antibodies were raised to rcIL-4 for use in an enzyme-linked immunosorbent assay (ELISA). This will facilitate studies on the role of cIL-4 in inflammatory diseases, particularly rheumatoid arthritis. (C) 2001 Academic Press.

引用

页码：88 / 92

页数：5

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