Biodistribution of an adenoviral vector carrying the luciferase reporter gene following intravesical or intravenous administration to a mouse

被引:104
作者
Wood, M
Perrotte, P
Onishi, E
Harper, ME
Dinney, C
Pagliaro, L
Wilson, DR
机构
[1] Introgen Therapeut Inc, Houston, TX 77054 USA
[2] Univ Texas, MD Anderson Canc Ctr, Houston, TX 77030 USA
关键词
biodistribution; AdLuc; polymerase chain reaction; luciferase activity; infravesical administration; tail vein administration;
D O I
10.1038/sj.cgt.7700090
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The biodistribution and resulting pattern of transgene expression were determined following intravesical administration of an adenoviral vector carrying the luciferase reporter gene (AdLuc). Female BALB/c mice were subjected to intravesical instillation of 1 x 10(9) or 5 x 10(9) plaque-forming units of AdLuc. After sacrifice, transgene expression was detected in tissues using luciferase assays; vector DNA was detected by vector-specific polymerase chain reaction. These experiments showed very little vector dissemination outside of the bladder by this route of administration. High-level expression of the vector transgene in the bladder was found to diminish by severalfold after 3 days. In a supporting study, vector dissemination and resulting transgene expression were determined following tail vein injection of 5 x 10(9) plaque-forming units of AdLuc. Vector was distributed to and expressed in every organ analyzed, with the highest concentration and level of expression observed in the liver.
引用
收藏
页码:367 / 372
页数:6
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