Enhancement of antibacterial effects of epigallocatechin gallate, using ascorbic acid

被引：49

作者：

Hatano, Tsutomu ^{[1
]}

Tsugawa, Mayumi ^{[1
]}

Kusuda, Miwako ^{[1
]}

Taniguchi, Shoko ^{[2
]}

Yoshida, Takashi ^{[1
]}

Shiota, Sumiko ^{[3
]}

Tsuchiya, Tomofusa ^{[4
]}

机构：

[1] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Pharmacognosy, Okayama 7008530, Japan

[2] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Med Bot Garden, Okayama 7008530, Japan

[3] Shujitu Univ, Sch Pharm, Dept Pathogen Microbiol, Okayama 7038516, Japan

[4] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Microbiol, Okayama 7008530, Japan

来源：

PHYTOCHEMISTRY | 2008年 / 69卷 / 18期

关键词：

Camellia sinensis L. (Theaceae); Antibacterial; Antibiotic resistance; Ascorbic acid; Epigallocatechin gallate; Methicillin-resistant Staphylococcus aureus; Polyphenol; Tea;

D O I：

10.1016/j.phytochem.2007.08.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Although plant polyphenols such as (-)-epigallocatechin gallate (EGCG) have antibacterial activity towards methicillin-resistant Staphylococcus aureus (MRSA), such polyphenols are unstable in solution. Because the instability of polyphenols is attributable to their oxidation, we examined the effects of antioxidants and inhibitors of polyphenol oxidation on the maintenance of polyphenol antibacterial activity. The antibacterial activity of EGCG was enhanced in the presence of ascorbic acid, and ascorbic acid was the most effective for retaining the concentration of stable EGCG. On the other hand, the antibacterial activity of EGCG was lowered in the presence of casein in spite of its suppressing effect on the EGCG decrease. The effect of EGCG on the antibiotic resistance of MRSA was also enhanced in the presence of ascorbic acid. The addition of an antioxidant may affect other pharmacological effects of polyphenols in analogous ways, although this does not mean the clinical usefulness of the addition directly. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：3111 / 3116

页数：6

共 9 条

[1] Community-acauired resistant Staphylococcus aureus skin infection methicillin [J].