Intercellular exchange of class II major histocompatibility complex/peptide complexes is a conserved process that requires activation of T cells but is constitutive in other types of antigen presenting cell

被引:27
作者
Patel, DM [1 ]
Mannie, MD [1 ]
机构
[1] E Carolina Univ, Sch Med, Dept Microbiol & Immunol, Greenville, NC 27858 USA
关键词
antigen presentation; cell-to-cell interactions; MHC; T-cell receptors; T lymphocyte;
D O I
10.1006/cimm.2001.1897
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Activated T cells acquire antigen presenting cell-(APC) derived class II major histocompatibility complex glycoproteins (MHCII) but the role of TCR in this process is controversial. This study provides additional evidence that ligation of TCR initiates activation-dependent processes that independently mediate acquisition of APC-derived molecules. First, intercellular exchange of MHCII resulted in the constitutive accumulation of xenogeneic rat I-A on murine B cells, whereas naive murine T cells required activation to adsorb xenogeneic I-A. Likewise, continuous lines of B cells, basophils, and MO from various species such as rat, mouse, and human constitutively acquired xenogeneic I-A. Second, inhibitors of T-cell activation such as wortmannin, EGTA, or mAb against I-A, TCR, LFA-1, or CD4 inhibited I-A acquisition by rested T cells but not by preactivated T cells. In conclusion, exchange of MHCII is a conserved process that requires activation of T cells but is constitutive in other types of APC. (C) 2001 Elsevier Science (USA).
引用
收藏
页码:165 / 172
页数:8
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