Expression of embryonic fibronectin isoform EIIIA parallels α-smooth muscle actin in maturing and diseased kidney

被引:23
作者
Barnes, VL
Musa, J
Mitchell, RJ
Barnes, JL
机构
[1] Univ Texas, Hlth Sci Ctr, Dept Med, Div Nephrol, San Antonio, TX 78284 USA
[2] Audie L Murphy Mem Vet Hosp, Med Res Serv, San Antonio, TX 78284 USA
[3] Wilford Hall USAF Hosp, Dept Nephrol, San Antonio, TX USA
关键词
fibronectin; alternative splicing; smooth muscle actin; mesangium; glomerulonephritis; interstitial nephritis; fibrosis;
D O I
10.1177/002215549904700608
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In this study we examined if an association exists between expression of an alternatively spliced "embryonic" fibronectin isoform EIIIA (Fn-EIIIA) and or-smooth muscle actin (alpha-SMA) in the maturing and adult rat kidney and in two unrelated models of glomerular disease, passive accelerated anti-glomerular basement membrane (GBM) nephritis and Habu venom (HV)-induced proliferative glomerulonephritis, using immunohistochemistry and in situ hybridization. Fn-EIIIA and alpha-SMA proteins were abundantly expressed in mesangium and in periglomerular and peritubular interstitium of 20-day embryonic and 7-day (D-7) postnatal kidneys in regions of tubule and glomerular development. Staining was markedly reduced in these structures in maturing juvenile (D-14) kidney and was largely lost in adult kidney. Expression of Fn-EIIIA and alpha-SMA was reinitiated in the mesangium and the periglomerular and peritubular interstitium in both models and was also observed in glomerular crescents in anti-GBM nephritis. Increased expression of Fn-EIIIA mRNA by in situ hybridization corresponded to the localization of protein staining. Dual labeling experiments verified co-localization of Fn-EIIIA and alpha-SMA, showing a strong correlation of staining between location and staining intensity during kidney development, maturation, and disease. Expression of EIIIA mRNA corresponded to protein expression in developing and diseased kidneys and was lost in adult kidney. These studies show a recapitulation of the co-expression of Fn-EIIIA and alpha-SMA in anti-GBM disease and suggest a functional link for these two proteins.
引用
收藏
页码:787 / 797
页数:11
相关论文
共 56 条
  • [1] Glomerular up-regulation of EIIIA and V120 fibronectin isoforms in proliferative immune complex nephritis
    Alonso, J
    GomezChiarri, M
    Ortiz, A
    Seron, D
    Condom, E
    LopezArmada, MJ
    Largo, R
    Barat, A
    Egido, J
    [J]. KIDNEY INTERNATIONAL, 1996, 50 (03) : 908 - 919
  • [2] ENHANCED EXPRESSION OF MUSCLE-SPECIFIC ACTIN IN GLOMERULONEPHRITIS
    ALPERS, CE
    HUDKINS, KL
    GOWN, AM
    JOHNSON, RJ
    [J]. KIDNEY INTERNATIONAL, 1992, 41 (05) : 1134 - 1142
  • [3] ALPERS CE, 1996, KIDNEY INT S54, V49, P28
  • [4] Atkins RC, 1996, J AM SOC NEPHROL, V7, P2271
  • [5] BARNES JL, 1995, AM J PATHOL, V147, P1361
  • [6] BARNES JL, 1994, LAB INVEST, V70, P460
  • [7] GLOMERULAR LOCALIZATION OF PLATELET SECRETORY PROTEINS IN MESANGIAL PROLIFERATIVE LESIONS INDUCED BY HABU SNAKE-VENOM
    BARNES, JL
    [J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1989, 37 (07) : 1075 - 1082
  • [8] BARNES JL, 1994, AM J PATHOL, V145, P585
  • [9] BARNES JL, 1991, AM J PATHOL, V138, P859
  • [10] BARNES JL, 1993, AM J PATHOL, V143, P1366