Laser autofocusing system for high-resolution cell biological imaging

被引：58

作者：

Liron, Y ^{[1
]}

Paran, Y ^{[1
]}

Zatorsky, NG ^{[1
]}

Geiger, B ^{[1
]}

Kam, Z ^{[1
]}

机构：

[1] Weizmann Inst Sci, IL-76100 Rehovot, Israel

来源：

JOURNAL OF MICROSCOPY | 2006年 / 221卷

关键词：

D O I：

10.1111/j.1365-2818.2006.01550.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Automated acquisition of high resolution, light microscope images of cells is becoming a common requirement in modern proteomic and cellomic research. A prerequisite for such microscopy is fine focus tuning, commonly optimized by multiple exposures, followed by image sharpness analysis. We describe here an extremely fast and accurate laser autofocusing system with distinct advantages for large-scale cell-based screening.

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页码：145 / 151

页数：7

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