β-Arrestin is involved in the desensitization but not in the internalization of the somatostatin receptor 2A expressed in CHO cells

被引：26

作者：

Brasselet, S ^{[1
]}

Guillen, S ^{[1
]}

Vincent, JP ^{[1
]}

Mazella, J ^{[1
]}

机构：

[1] CNRS, Inst Pharmacol Mol & Cellulaire, UMR 6097, F-06560 Valbonne, France

来源：

FEBS LETTERS | 2002年 / 516卷 / 1-3期

关键词：

somatostatin; receptor; beta-arrestin; internalization; desensitization;

D O I：

10.1016/S0014-5793(02)02517-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction of beta-arrestin-1 with the somatostatin receptor type 2A (sst2A) was monitored using both biochemical and confocal imaging approaches. We show that, using transient transfection of either beta-arrestin-1 or its dominant negative Delta-arrestin-1 in CHO cells stably transfected with the sst2A, beta-arrestin-1 is colocalized with the receptor in endosomal vesicles after somatostatin-induced sequestration. However, this interaction leads to a role of beta-arrestin-1 in the desensitization of the sst2A rather than in the internalization process of the receptor-ligand complex. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：124 / 128

页数：5

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