Thermally controlled protein release from gelatin-dextran hydrogels

被引：13

作者：

Aso, Y ^{[1
]}

Yoshioka, S ^{[1
]}

Nakai, Y ^{[1
]}

Kojima, S ^{[1
]}

机构：

[1] Natl Inst Hlth Sci, Tokyo 158, Japan

来源：

RADIATION PHYSICS AND CHEMISTRY | 1999年 / 55卷 / 02期

关键词：

D O I：

10.1016/S0969-806X(98)00324-7

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Biodegradable hydrogels in which drug release was controlled by sol-gel transition were prepared. Gelatin was used as a component because it exhibits sol-gel transition in response to temperature changes. Glycidyl methacrylated (GMA) dextran was crosslinked by low dose gamma-irradiation in the presence of gelatin and the model drugs, beta-galactosidase (beta-GA), bovine serum albumin (BSA) or 5-fluorouracil (5-FU). The enzyme activity of beta-GA remained greater than 95% after irradiation. Temperature-responsive release of beta-GA and BSA resulted from the sol-gel transition of gelatin. Sol-gel transition was confirmed by the temperature dependence of the spin-spin relaxation time of the gel polymer protons. The protein release rate was affected by both the degree of GMA substitution and the gelatin concentration. Desired release rate could be achieved by adjusting these factors. The release rate of 5-FU was not affected by the sol-gel transition of gelatin. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：179 / 183

页数：5

共 11 条

[1] Pulsatile local delivery of thrombolytic and antithrombotic agents using poly(N-isopropylacrylamide-co-methacrylic acid) hydrogels [J].