Temporal profile of serum-induced S-phase entry and retinoblastoma protein phosphorylation in human skin fibroblasts

被引：8

作者：

Danesh, FR

Ye, MH

Salmi, S

LaPointe, M

Batlle, D

机构：

[1] Northwestern Univ, Sch Med, Div Nephrol Hypertens, Chicago, IL 60611 USA

[2] Chicago VA Healthcare Syst, Lakeside Div, Chicago, IL USA

来源：

KIDNEY INTERNATIONAL | 1999年 / 56卷 / 04期

关键词：

hyperplastic growth phenotype; glomerular mesangial cells; cell growth; type; 1; diabetes; nephropathy;

D O I：

10.1046/j.1523-1755.1999.00690.x

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

Cultured skin fibroblasts from type 1 diabetic patients with nephropathy have a hyperplastic growth phenotype in comparison with diabetics without nephropathy and controls. We studied the G1 phase cyclins in skin fibroblasts from control subjects to define the temporal profile of serum-induced pRB phosphorylation, cyclin D1 protein expression, and cyclin D1/CDK4(6) kinase activity as well as S-phase entry by FAGS analysis. Our preliminary studies indicate that cultured skin fibroblasts from type 1 diabetic patients with nephropathy have an enhanced PRE phosphorylation, cyclin D1 protein expression, and cyclin D1/CDK4(6) kinase activity. This finding may become useful to identify patients at risk for the development of nephropathy.

引用

页码：1282 / 1285

页数：4

共 8 条

[1]

KATO J, 1993, GENE DEV, V7, P331

[2] Growth phenotype of cultured skin fibroblasts from IDDM patients with and without nephropathy and overactivity of the Na+/H+ antiporter [J].