Simultaneous identification of A, B, D and R genomes by genomic in situ hybridization in wheat-rye derivatives

被引：28

作者：

Sánchez-Morán, E ^{[1
]}

Benavente, E ^{[1
]}

Orellana, J ^{[1
]}

机构：

[1] Univ Politecn Madrid, ETSI Agron, Dept Biotecnol, Unidad Genet, E-28040 Madrid, Spain

来源：

HEREDITY | 1999年 / 83卷 / 3期

关键词：

genome identification; in situ hybridization; multicolour fluorescence; wheat-rye derivatives;

D O I：

10.1038/sj.hdy.6885570

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

Multicolour genomic in situ hybridization was carried out in wheat-rye hybrids and in a wheat-rye translocation line. Different hybridization conditions and mixture compositions were used, and A, B and D genomes of hexaploid wheat as well as the R genome of rye were distinguished simultaneously in somatic cells. Combination of genomic and rDNA probes in multicolour in situ hybridization was also performed to identify chromosomes within a specific genome.

引用

页码：249 / 252

页数：4

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