Free fatty acid formation and lipid oxidation on milled rice

Milled rice was stored at 37degreesC and 70% humidity and sampled regularly for 50 d. Rice surface lipid was extracted with isopropanol and analyzed for free fatty acids (FFA) and conjugated diene (CD) contents. Diffuse reflectance Fourier transform infrared (DRIFTS) spectra of the rice samples were also obtained. FFA and CD levels increased together during rice storage and exhibited three distinct phases. DRIFTS identified a decrease in intensity at 1746 cm(-1) (ester, -C=O) and increases in intensity at 1731 cm-1 (aldehyde, -CO) and 1714 cm(-1) (fatty acid, -C=O) during storage, which correlated well with the chemical analysis data. DRIFTS spectral data were analyzed by a partial least squares regression method to identify spectral regions that correlate strongly with measured FFA and construct prediction models. Overall, the mid-infrared region (4000-400 cm(-1)) gave the best model (R = 0.98, root mean square error of cross-validation = 0.05) and also predicted the FFA content of milled rice well. The DRIFTS technique has potential for use in studying qualitative chemical changes on the milled rice surface lipids and for predicting FFA on milled rice.