Polymerase chain reaction assay to determine infection of Aedes polynesiensis by Wuchereria bancrofti

被引：32

作者：

Nicolas, L ^{[1
]}

Luquiaud, P ^{[1
]}

Lardeux, F ^{[1
]}

Mercer, DR ^{[1
]}

机构：

[1] ORSTOM, INST FRANCAIS RECH SCI DEV COOPERAT, PAPEETE, TAHITI, FRANCE

来源：

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE | 1996年 / 90卷 / 02期

关键词：

filariasis; Wuchereria bancrofti; Aedes polynesiensis; polymerase chain reaction;

D O I：

10.1016/S0035-9203(96)90113-3

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

The sensitivity of a previously described polymerase chain reaction (PCR) assay was improved to detect a single mosquito, infected by as few as 1-2 microfilariae of Wuchereria bancrofti, among 20-50 uninfected mosquitoes. Wild-caught Aedes polynesiensis were used to compare assessment of infection by dissection of individuals with the PCR assay of pools of mosquitoes. The PCR assay was at least as sensitivite as dissection for detection of mosquitoes infected with W. bancrofti.

引用

页码：136 / 139

页数：4

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