BEAMing up for detection and quantification of rare sequence variants

被引：198

作者：

Li, M

Diehl, F

Dressman, D

Vogelstein, B

Kinzler, KW

机构：

[1] Johns Hopkins Univ, Sch Med, Sidney Kimmel Comprehens Canc Ctr, Howard Hughes Med Inst, Baltimore, MD 21231 USA

[2] Johns Hopkins Univ, Sch Med, Dept Pharmacol & Mol Sci, Baltimore, MD 21231 USA

来源：

NATURE METHODS | 2006年 / 3卷 / 02期

关键词：

D O I：

10.1038/NMETH850

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

BEAMing allows the one-to-one conversion of a population of DNA fragments into a population of beads. We used rotting circle amplification to increase the number of copies bound to such beads by more than 100-fold. This allowed enumeration of mutant and wild-type sequences even when they were present at ratios less than 1:10,000 and was sensitive enough to directly quantify the error rate of DNA polymerases used for PCR.

引用

页码：95 / 97

页数：3

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