Antagonism Versus Cooperativity with TALE Cofactors at the Base of the Functional Diversification of Hox Protein Function

被引:26
作者
Luisa Rivas, Maria [1 ]
Manuel Espinosa-Vazquez, Jose [1 ]
Sambrani, Nagraj [2 ]
Greig, Stephen [3 ]
Merabet, Samir [2 ]
Graba, Yacine [2 ]
Castelli-Gair Hombria, James [1 ]
机构
[1] Univ Pablo de Olavide, CABD, CSIC, JA, Seville, Spain
[2] Univ Aix Marseille 2, IBDML, CNRS, Marseille, France
[3] Univ Cambridge, Akam Lab, Cambridge, England
来源
PLOS GENETICS | 2013年 / 9卷 / 02期
关键词
DNA-BINDING SPECIFICITY; HOMEOTIC GENE-FUNCTION; ABDOMINAL-B GENE; BITHORAX COMPLEX; HOMEODOMAIN PROTEINS; INDUCED ORGANOGENESIS; REGULATORY FUNCTIONS; POSTERIOR SPIRACLES; TARGET GENE; DROSOPHILA;
D O I
10.1371/journal.pgen.1003252
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Extradenticle (Exd) and Homothorax (Hth) function as positive transcriptional cofactors of Hox proteins, helping them to bind specifically their direct targets. The posterior Hox protein Abdominal-B (Abd-B) does not require Exd/Hth to bind DNA; and, during embryogenesis, Abd-B represses hth and exd transcription. Here we show that this repression is necessary for Abd-B function, as maintained Exd/Hth expression results in transformations similar to those observed in loss-of-function Abd-B mutants. We characterize the cis regulatory module directly regulated by Abd-B in the empty spiracles gene and show that the Exd/Hth complex interferes with Abd-B binding to this enhancer. Our results suggest that this novel Exd/Hth function does not require the complex to bind DNA and may be mediated by direct Exd/Hth binding to the Abd-B homeodomain. Thus, in some instances, the main positive cofactor complex for anterior Hox proteins can act as a negative factor for the posterior Hox protein Abd-B. This antagonistic interaction uncovers an alternative way in which MEIS and PBC cofactors can modulate Abd-B like posterior Hox genes during development.
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页数:14
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