Ordered differential display: A simple method for systematic comparison of gene expression profiles

被引：64

作者：

Matz, M ^{[1
]}

Usman, N ^{[1
]}

Shagin, D ^{[1
]}

Bogdanova, E ^{[1
]}

Lukyanov, S ^{[1
]}

机构：

[1] RUSSIAN ACAD SCI,SHEMYAKIN & OVCHINNIKOV INST BIOORGAN CHEM,MOSCOW 117871,RUSSIA

来源：

NUCLEIC ACIDS RESEARCH | 1997年 / 25卷 / 12期

关键词：

D O I：

10.1093/nar/25.12.2541

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for display of 3'-end restriction fragments of cDNAs is proposed, extending the idea reported recently. First, representative pools of such fragments are selectively amplified using PCR suppression effect. Then, simplified subsets of these fragments suitable for comparison by PAGE are amplified by adapter-specific primers extended by two randomly picked bases at their 3' ends. By testing all possible combinations of extended primers the whole mRNA pool may be systematically investigated. The method was applied to search for molecular regional markers of freshwater planarian Dugesia tigrina.

引用

页码：2541 / 2542

页数：2

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