Differential expression of two spermidine synthase genes during early fruit development and in vegetative tissues of pea

被引：29

作者：

Alabadí, D ^{[1
]}

Carbonell, J ^{[1
]}

机构：

[1] Univ Politecn Valencia, CSIC, Inst Biol Mol & Celular Plantas, Valencia 46022, Spain

来源：

PLANT MOLECULAR BIOLOGY | 1999年 / 39卷 / 05期

关键词：

cloning; fruit development; gene expression; pea; polyamine; spermidine synthase;

D O I：

10.1023/A:1006158819849

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two cDNAs from young pea fruits coding for functional spermidine synthases (EC 2.5.1.16) were isolated. The corresponding genes were named psSPDSYN1 and psSPDSYN2. Both cDNAs complemented spe3 Delta gene when introduced into the Y480 strain of Saccharomyces cerevisiae, which is a null mutant for the spermidine synthase gene. psSPDSYN1 and psSPDSYN2 are regulated differentially. psSPDSYNI is up-regulated early after fruit set whereas psSPDSYN2 is expressed later. Spermidine synthase activity was detected in pea ovaries, and correlates with the pattern of expression of psSPDSYN1. In the pea plant, psSPDSYNI is highly expressed in actively growing tissues, whereas the highest level of psSPDSYN2 mRNA was detected in fully elongated stem.

引用

页码：933 / 943

页数：11

共 41 条

[1] Arginase, arginine decarboxylase, ornithine decarboxylase and polyamines in tomato ovaries - Changes in unpollinated ovaries and parthenocarpic fruits induced by auxin or gibberellin [J].

Alabadi, D ;

Aguero, MS ;

PerezAmador, MA ;

Carbonell, J .

PLANT PHYSIOLOGY, 1996, 112 (03) :1237-1244

[2]

ALABADI D, IN PRESS PLANT PHYSL

[3] Putrescine aminopropyltransferase is responsible for biosynthesis of spermidine, spermine, and multiple uncommon polyamines in osmotic stress-tolerant alfalfa [J].

Bagga, S ;

Rochford, J ;

Klaene, Z ;

Kuehn, GD ;

Phillips, GC .

PLANT PHYSIOLOGY, 1997, 114 (02) :445-454

[4] Different sequences for 5'-untranslated leaders of nuclear genes for plastid proteins affect the expression of the beta-glucuronidase gene [J].

Bolle, C ;

Herrmann, RG ;

Oelmuller, R .

PLANT MOLECULAR BIOLOGY, 1996, 32 (05) :861-868

[5]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[6] CORRELATION OF SPERMINE LEVELS WITH OVARY SENESCENCE AND WITH FRUIT-SET AND DEVELOPMENT IN PISUM-SATIVUM-L [J].

CARBONELL, J ;

NAVARRO, JL .

PLANTA, 1989, 178 (04) :482-487

[7] FRUIT-SET OF UN-POLLINATED OVARIES OF PISUM-SATIVUM-L - INFLUENCE OF VEGETATIVE PARTS [J].

CARBONELL, J ;

GARCIAMARTINEZ, JL .

PLANTA, 1980, 147 (05) :444-450

[8]

Dellaporta S.L., 1983, Plant Molecular Biology Reporter, V1, P19, DOI DOI 10.1007/BF02712670

[9]

Egea-Cortines M., 1991, BIOCH PHYSL POLYAMIN, P143

[10]

EGEACORTINES M, 1993, PHYSIOL PLANTARUM, V87, P14, DOI [10.1034/j.1399-3054.1993.870103.x, 10.1111/j.1399-3054.1993.tb08784.x]

← 1 2 3 4 5 →