Direct determination of tramadol glucuronides in human urine by high-performance liquid chromatography with fluorescence detection

被引:36
作者
Overbeck, P [1 ]
Blaschke, G [1 ]
机构
[1] Univ Munster, Inst Pharmaceut Chem, D-48149 Munster, Germany
来源
JOURNAL OF CHROMATOGRAPHY B | 1999年 / 732卷 / 01期
关键词
tramadol glucuronide;
D O I
10.1016/S0378-4347(99)00281-9
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A sensitive and selective reversed-phase high-performance liquid chromatography method has been developed for the direct determination of three glucuronides of the centrally acting analgesic tramadol (1). Separation of these glucuronides into their diastereomers was achieved by HPLC using ion pair chromatography with nonanesulfonic acid sodium salt and LiChrospher 100 RP 18 as stationary phase. Quantification of O-demethyltramadol glucuronide and N,O-didemethyltramadol glucuronide in human urine was performed by fluorescence detection. The urine samples were purified by a two-step solid-phase extraction. The glucuronides were found to be highly enriched in the 1S,2S-diastereomers. The results of a study with three healthy volunteers are presented. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:185 / 192
页数:8
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