The Na+/e(-) stoichiometry of the Na+-motive NADH:quinone oxidoreductase in Vibrio alginolyticus

被引：49

作者：

Bogachev, AV ^{[1
]}

Murtazina, RA ^{[1
]}

Skulachev, VP ^{[1
]}

机构：

[1] MOSCOW MV LOMONOSOV STATE UNIV,AN BELOZERSKY INST PHYS CHEM BIOL,DEPT BIOENERGET,MOSCOW 119899,RUSSIA

来源：

FEBS LETTERS | 1997年 / 409卷 / 03期

基金：

俄罗斯基础研究基金会;

关键词：

Na+/e(-) stoichiometry; Na+-motive NADH:quinone oxidoreductase; Vibrio alginolyticus;

D O I：

10.1016/S0014-5793(97)00536-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is proposed to estimate the stoichiometries of primary Na+-pumps in intact bacterial cells, It is based on technique when the H+/e(-) stoichiometry is measured in the presence of protophorous uncoupler and in the absence of penetrating ions other than H+, Under these conditions, the H+ influx discharges membrane potential generated by the Na+ pump so the Na+/e(-) and H+/e(-) ratios become equal. Using this approach it is shown that the Na+/e(-) ratio for the Na+-motive NADH : quinone oxidoreductase of Vibrio alginolyticus is equal to 0.71+/-0.06. The Na+/e(-) stoichiometry appears to be approximate to 1, provided that the contribution of the non-coupled NADH : quinone oxidoreductase, which is resistant to low HQNO concentrations, is taken into account. (C) 1997 Federation of European Biochemical Societies.

引用

页码：475 / 477

页数：3

共 16 条

[11]

SKULACHEV VP, 1989, MEMBRANE BIOENERGETI

[12] DELTA-PSI AND DELTA-PH GENERATION BY THE H+ PUMPS OF THE RESPIRATORY-CHAIN AND ATPASE IN SUBCELLULAR VESICLES FROM MARINE BACTERIUM VIBRIO-ALGINOLYTICUS [J].

SMIRNOVA, IA ;

VAGHINA, ML ;

KOSTYRKO, VA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1016 (03) :385-391

[13]

TOKUDA H, 1982, J BIOL CHEM, V257, P788

[14]

TOKUDA H, 1984, J BIOL CHEM, V259, P7785

[15]

TOKUDA H, 1982, J BIOL CHEM, V257, P7

[16] CRITICAL-EVALUATION OF THE PROTON TRANSLOCATING PROPERTY OF CYTOCHROME-OXIDASE IN RAT-LIVER MITOCHONDRIA [J].

WIKSTROM, M ;

PENTTILA, T .

FEBS LETTERS, 1982, 144 (02) :183-189

← 1 2 →