Expression of c-myc is down-regulated as mouse mammary epithelial cells become confluent

被引:11
作者
Grolli, S [1 ]
Accornero, P [1 ]
Ramoni, R [1 ]
Donofrio, G [1 ]
Whitelaw, CBA [1 ]
机构
[1] ROSLIN INST,DIV MOL BIOL,ROSLIN EH25 9PS,MIDLOTHIAN,SCOTLAND
基金
英国生物技术与生命科学研究理事会;
关键词
D O I
10.1006/bbrc.1997.7498
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have investigated the expression profile of c-mye in the mammary gland, During pregnancy when the gland is actively growing c-myc mRNA was present, while in the differentiated lactating gland no c-myc mRNA was detected. This correspondence between the differentiation state and c-mye mRNA levels in the mouse mammary gland in viva was paralleled by HC11 mouse mammary epithelial cells in vitro. Firstly, the endogenous c-mye gene was suppressed in confluent compared to growing HC11 cells. in addition, treating the cells with lactogenic hormones did not induce c-myc expression. Secondly, a stably transfected c-myc-CAT reporter construct was similarly down-regulated, Furthermore, using this transfection model, we demonstrate that the mechanism(s) involved in regulating c-myc expression must act through the P1 and P2 core promoter and exon 1. Finally, we demonstrate that suppression of c-myc expression occurs when HC11 cells growth-arrest as they become confluent. (C) 1997 Academic Press.
引用
收藏
页码:566 / 569
页数:4
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