Improved method for plasma malondialdehyde measurement by high-performance liquid chromatography using methyl malondialdehyde as an internal standard

被引：120

作者：

Sim, AS ^{[1
]}

Salonikas, C ^{[1
]}

Naidoo, D ^{[1
]}

Wilcken, DEL ^{[1
]}

机构：

[1] Prince Wales Hosp, Dept Med, Cardiovasc Genet Lab, Sydney, NSW 2031, Australia

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2003年 / 785卷 / 02期

关键词：

malondialdehyde;

D O I：

10.1016/S1570-0232(02)00956-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Measurement of malondialdehyde (MDA) is an important contribution to the assessment of oxidative stress. We report a sensitive and reproducible high-performance liquid chromatography (HPLC) method for measurement of plasma MDA in the assessment of lipid peroxidation. Using methyl malondialdehyde (Me-MDA) as an internal standard with reversed-phase HPLC and UV detection and derivatisation with 2,4 dinitrophenylhydrazine (DNPH), we obtained maximum MDA values with 60-min incubation of 10% plasma with 1 M NaOH at 60 degreesC. The dilution of the plasma and a longer incubation time in the alkaline hydrolysis step greatly improved recovery of MDA from its bound form. Ratios of peak height of MDA/Me-MDA were linear over a range of 0-100 muM with correlation coefficients >0.99. The recovery was 88.5%. Within and between run variations were <4 and <7%, respectively. The mean MDA value measured in 20 healthy volunteers was 13.8 muM (+/-1.32). (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：337 / 344

页数：8

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