Ribozymes, the first 20 years

被引：86

作者：

Cech, TR

机构：

[1] Howard Hughes Med Inst, Chevy Chase, MD 20815 USA

[2] Univ Colorado, Boulder, CO 80309 USA

来源：

BIOCHEMICAL SOCIETY TRANSACTIONS | 2002年 / 30卷

关键词：

catalytic RNA; group I intron; RNA structure; splicing; X-ray crystallography;

D O I：

10.1042/BST0301162

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In 1982 we reported the first catalytic RNA or ribozyme: the self-splicing intron of the Tetrahymena pre-rRNA. Additional examples of natural ribozymes were soon found, and research in the field focused on their enzymic mechanism and secondary and tertiary structure. Ribozymes identified through in vitro selection extended the repertoire of RNA catalysis. Two directions of current and future interest are the determination of atomic-resolution structures of large ribozymes by X-ray crystallography and the structural and mechanistic analysis of complexes of ribozymes with protein facilitators of their activity.

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页码：1162 / 1166

页数：5

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