Dendritic sugar-microarrays by click chemistry

被引：36

作者：

Fukuda, Tomohiro ^{[1
]}

Onogi, Shunsuke ^{[1
]}

Miura, Yoshiko ^{[1
]}

机构：

[1] Japan Adv Inst Sci & Technol, Sch Mat Sci, Nomi, Ishikawa 9231292, Japan

来源：

THIN SOLID FILMS | 2009年 / 518卷 / 02期

关键词：

Click chemistry; Glycodendrimer; Lectin; Self-assembled monolayer; Surface plasmon resonance; CARBOHYDRATE-PROTEIN INTERACTIONS; INFRARED-SPECTROSCOPY; CONCANAVALIN-A; BINDING; DENDRIMERS; LECTIN; RECEPTOR; WATER; RECOGNITION; MECHANISMS;

D O I：

10.1016/j.tsf.2009.07.109

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Saccharide microarrays with well-defined multivalency were prepared using glycodendrimers. The glycodendrimers with various generations and saccharides (alpha-Man, beta-GlcNAc and P-Gal) were synthesized and immobilized on an acetylenyl-terminated gold substrate via click chemistry. The saccharide-immobilized substrates were analyzed using XPS, ellipsometry, cyclic voltammetry, contact angle goniometry, QCM and FTIR-RAS. The biological abilities of the saccharide-immobilized substrates were evaluated by SPR with the lectins of Con A, WGA and RCA(120). Lectin binding to the glycodendrimer array was quantitatively analyzed, and association constants (K-A) and kinetic rate constants (k(a) and k(d)) were evaluated. These kinetic constants were unique to each lectin, implying specificity of the multivalent effect of saccharide-lectin interaction. (C) 2009 Elsevier B.V. All rights reserved

引用

页码：880 / 888

页数：9

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