Heart failure alters matrix metalloproteinase gene expression and activity in rat skeletal muscle

被引：35

作者：

Carvalho, Robson Francisco ^{[1
]}

Dariolli, Rafael

Justulin Junior, Luis Antonio

Sugizaki, Mario Mateus

Okoshi, Marina Politi

Cicogna, Antonio Carlos

Felisbino, Sergio Luis

Dal Pai-Silva, Maeli

机构：

[1] Univ Estadual Paulista, Dept Morphol, BR-18618000 Botucatu, SP, Brazil

[2] Univ Estadual Campinas, Dept Biol Celular, Campinas, SP, Brazil

[3] Univ Estadual Paulista, Dept Clin Med, Botucatu, SP, Brazil

来源：

INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY | 2006年 / 87卷 / 06期

关键词：

extracellular matrix; heart failure; metalloproteinases; skeletal muscle; Wistar rats;

D O I：

10.1111/j.1365-2613.2006.00497.x

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Heart failure is associated with a skeletal muscle myopathy with cellular and extracellular alterations. The hypothesis of this investigation is that extracellular changes may be associated with enhanced mRNA expression and activity of matrix metalloproteinases (MMP). We examined MMP mRNA expression and MMP activity in Soleus (SOL), extensor digitorum longus (EDL), and diaphragm (DIA) muscles of young Wistar rat with monocrotaline-induced heart failure. Rats injected with saline served as age-matched controls. MMP2 and MMP9 mRNA contents were determined by RT-PCR and MMP activity by electrophoresis in gelatin-containing polyacrylamide gels in the presence of SDS under non-reducing conditions. Heart failure increased MMP9 mRNA expression and activity in SOL, EDL and DIA and MMP2 mRNA expression in DIA. These results suggest that MMP changes may contribute to the skeletal muscle myopathy during heart failure.

引用

页码：437 / 443

页数：7

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