Editing independent effects of ADARs on the miRNA/siRNA pathways

被引：154

作者：

Heale, Bret S. E. ^{[1
]}

Keegan, Liam P. ^{[1
]}

McGurk, Leeanne ^{[1
]}

Michlewski, Gracjan ^{[1
]}

Brindle, James ^{[1
]}

Stanton, Chloe M. ^{[1
]}

Caceres, Javier F. ^{[1
]}

O'Connell, Mary A. ^{[1
]}

机构：

[1] Western Gen Hosp, Inst Genet & Mol Med, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland

来源：

EMBO JOURNAL | 2009年 / 28卷 / 20期

基金：

英国医学研究理事会;

关键词：

Drosophila; DSH1; microprocessor; RNA editing; RNA interference; DOUBLE-STRANDED-RNA; DYSCHROMATOSIS SYMMETRICA HEREDITARIA; ADENOSINE-DEAMINASE; MESSENGER-RNA; GENE; BINDING; DROSOPHILA; MUTATIONS; INTERFERON; EXPRESSION;

D O I：

10.1038/emboj.2009.244

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Adenosine deaminases acting on RNA (ADARs) are best known for altering the coding sequences of mRNA through RNA editing, as in the GluR-B Q/R site. ADARs have also been shown to affect RNA interference (RNAi) and microRNA processing by deamination of specific adenosines to inosine. Here, we show that ADAR proteins can affect RNA processing independently of their enzymatic activity. We show that ADAR2 can modulate the processing of mir-376a2 independently of catalytic RNA editing activity. In addition, in a Drosophila assay for RNAi deaminase-inactive ADAR1 inhibits RNAi through the siRNA pathway. These results imply that ADAR1 and ADAR2 have biological functions as RNA-binding proteins that extend beyond editing per se and that even genomically encoded ADARs that are catalytically inactive may have such functions. The EMBO Journal (2009) 28, 3145-3156. doi:10.1038/sj.emboj.2009.244; Published online 27 August 2009

引用

页码：3145 / 3156

页数：12

共 55 条

[1] RNA hyperediting and alternative splicing of hematopoietic cell phosphatase (PTPN6) gene in acute myeloid leukemia [J].