Differential screening indicates a dramatic change in mRNA profiles during grape berry ripening. Cloning and characterization of cDNAs encoding putative cell wall and stress response proteins

被引:186
作者
Davies, C
Robinson, SP
机构
[1] Cooperat Res Ctr Viticulture, Glen Osmond, SA 5064, Australia
[2] CSIRO, Plant Ind Hort Res Unit, Glen Osmond, SA 5064, Australia
关键词
D O I
10.1104/pp.122.3.803
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We used differential screening to isolate ripening-associated cDNAs from a Shiraz grape (Vitis vinifera L.) berry cDNA library. A rapid increase in the mRNA levels of a number of cDNAs not present in unripe fruit occurred in grape berries at the onset of ripening. The putative translation products of some of these clones had homologs in other species that are involved in cell wall structure. These included four proline-rich proteins, a small protein that is similar to the non-catalytic, N-terminal domain of some pectin methylesterases, and two other glutamate-rich proteins. The remainder of the clones encoded putative stress response proteins. These included two thaumatin like proteins, a metallothionein, a transcription factor, a cytochrome P450 enzyme, and proteins induced by water, sugar, and/or cold stress in other species. Many of the homologs of the grape cDNAs thought to be involved in cell wall structure or stress-related responses also accumulate in a developmental manner in other plants. This may indicate that the grape mRNAs accumulate in response to stresses such as the storage of high concentrations of sugars and rapid cell expansion, or they may accumulate as part of the ripening developmental program.
引用
收藏
页码:803 / 812
页数:10
相关论文
共 58 条
[1]   Characterization of two cDNA clones for mRNAs expressed during ripening of melon (Cucumis melo L) fruits [J].
Aggelis, A ;
John, I ;
Karvouni, Z ;
Grierson, D .
PLANT MOLECULAR BIOLOGY, 1997, 33 (02) :313-322
[2]   Analysis of the expression of anthocyanin pathway genes in developing Vitis vinifera L cv Shiraz grape berries and the implications for pathway regulation [J].
Boss, PK ;
Davies, C ;
Robinson, SP .
PLANT PHYSIOLOGY, 1996, 111 (04) :1059-1066
[3]   Plant responses to water deficit [J].
Bray, EA .
TRENDS IN PLANT SCIENCE, 1997, 2 (02) :48-54
[4]   ISOLATION OF CDNA CLONES FOR GENES THAT ARE EXPRESSED DURING LEAF SENESCENCE IN BRASSICA-NAPUS - IDENTIFICATION OF A GENE ENCODING A SENESCENCE-SPECIFIC METALLOTHIONEIN-LIKE PROTEIN [J].
BUCHANANWOLLASTON, V .
PLANT PHYSIOLOGY, 1994, 105 (03) :839-846
[5]   AN UNUSUAL GROUP-2 LEA GENE FAMILY IN CITRUS RESPONSIVE TO LOW-TEMPERATURE [J].
CAI, QY ;
MOORE, GA ;
GUY, CL .
PLANT MOLECULAR BIOLOGY, 1995, 29 (01) :11-23
[6]  
CAWTHON DL, 1982, J AM SOC HORTIC SCI, V107, P1097
[7]   The isolation of a novel metallothionein-related cDNA expressed in somatic and zygotic embryos of Douglas-fir: Regulation by ABA, osmoticum, and metal ions [J].
Chatthai, M ;
Kaukinen, KH ;
Tranbarger, TJ ;
Gupta, PK ;
Misra, S .
PLANT MOLECULAR BIOLOGY, 1997, 34 (02) :243-254
[8]   MOLECULAR-CLONING AND CHARACTERIZATION OF 6 CDNAS EXPRESSED DURING GLUCOSE STARVATION IN EXCISED MAIZE (ZEA-MAYS L) ROOT-TIPS [J].
CHEVALIER, C ;
BOURGEOIS, E ;
PRADET, A ;
RAYMOND, P .
PLANT MOLECULAR BIOLOGY, 1995, 28 (03) :473-485
[9]   Molecular cloning of a metallothionein-like gene from Nicotiana glutinosa L and its induction by wounding and tobacco mosaic virus infection [J].
Choi, D ;
Kim, HM ;
Yun, HK ;
Park, JA ;
Kim, WT ;
Bok, SH .
PLANT PHYSIOLOGY, 1996, 112 (01) :353-359
[10]   Differential gene expression in ripening banana fruit [J].
Clendennen, SK ;
May, GD .
PLANT PHYSIOLOGY, 1997, 115 (02) :463-469