Saccharospirillum salsuginis sp nov., a gammaproteobacterium from a subterranean brine

被引:12
作者
Chen, Yi-Guang [1 ,2 ]
Cui, Xiao-Long [1 ]
Li, Qin-Yuan [3 ]
Wang, Yong-Xia [1 ]
Tang, Shu-Kun [1 ]
Liu, Zhu-Xiang [1 ,2 ]
Wen, Meng-Liang [1 ]
Peng, Qian [1 ]
Xu, Li-Hua [1 ]
机构
[1] Yunnan Univ, Yunnan Inst Microbiol, Kunming 650091, Yunnan, Peoples R China
[2] Jishou Univ, Key Lab Conservat & Utilizat Plant Resources Huna, Coll Bioresources & Environm Sci, Jishou 416000, Hunan, Peoples R China
[3] Yunnan Univ, Expt Ctr Life Sci, Kunming 650091, Yunnan, Peoples R China
基金
中国国家自然科学基金;
关键词
GEN; NOV; DEOXYRIBONUCLEIC-ACID; BACTERIAL SYSTEMATICS; SEQUENCE ALIGNMENT; CELL-WALL; MARINE; TREES;
D O I
10.1099/ijs.0.003616-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A novel Gram-negative, non-sporulating, moderately halophilic, facultatively alkaliphilic, catalase- and oxidase-positive, obligately aerobic bacterium, strain YIM-Y25(T), was isolated from a subterranean brine sample collected from a salt mine in Yunnan, south-west China. Cells were spirilla, motile by monopolar flagella, with meso-diaminopimelic acid in the cell-wall peptidoglycan. Growth occurred with 1-15% (w/v) NaCl (optimum 5%), at pH 6.0-10.0 (optimum pH 8.0) and at 15-50 C degrees (optimum 35-40 degrees C). Ubiquinone 0-8 was the predominant respiratory quinone. Polar lipids were diphosphaticlylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylamine and an unidentified phospholipid. The major cellular fatty acids were C-18:1 omega 7c, iso-C-16:0, C-16:0 and C-16:1. The genomic DNA G + C content was 58.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM-Y25(T) was most closely related to the type strain of the sole recognized species of the genus Saccharospirillum, Saccharospirillum impatiens EL-105(T) (sequence similarity 97.0%), and these two strains formed a robust lineage in the phylogenetic tree. The level of DNA-DNA relatedness between them was 12.6%. The combination of phylogenetic analysis, phenotypic differences, chemotaxonomic properties and DNA-DNA hybridization data supported the view that this strain represents a novel species of the genus Saccharospirillum, for which the name Saccharospirillum salsuginis sp. nov. is proposed, with YIM-Y25(T) (=CCTCC AA 207033(T) =KCTC 22169(T)) as the type strain.
引用
收藏
页码:1382 / 1386
页数:5
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