Transfection of malaria parasites

被引：114

作者：

Waters, AP ^{[1
]}

Thomas, AW ^{[1
]}

vanDijk, MR ^{[1
]}

Janse, CJ ^{[1
]}

机构：

[1] BIOMED PRIMATE RES CTR, DEPT PARASITOL, NL-2288 GJ RIJSWIJK, NETHERLANDS

来源：

METHODS-A COMPANION TO METHODS IN ENZYMOLOGY | 1997年 / 13卷 / 02期

关键词：

D O I：

10.1006/meth.1997.0506

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The stable genetic transformation of three phylogenetically diverse species of Plasmodium, the parasitic etiological agent of malaria, is now possible. The parasite is haploid throughout the vast majority of its life cycle. Therefore with the single selectable marker activity and protocols currently available, it is possible not only to express introduced transgenes but also to study the effects of site-specific homologous recombination such as gene knockout. Transgene expression will allow the detailed study of many aspects of the cellular biology of malaria parasites, for example, the mechanisms underlying drug resistance and protein trafficking. We describe here the methods for propagation of the two animal models (Plasmodium berghei and Plasmodium knowlesi) and for transfection of these two species and the human parasite, Plasmodium falciparum. Examples of transgene expression are given. (C) 1997 Academic Press.

引用

页码：134 / 147

页数：14

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