4,7-phenanthroline-5,6-dione: A new labelling reagent for liquid chromatographic analysis of amino acids

被引：10

作者：

Gioia, MG ^{[1
]}

Gatti, R ^{[1
]}

Vannini, M ^{[1
]}

Hudaib, M ^{[1
]}

机构：

[1] Univ Bologna, Dipartimento Sci Farmaceut, I-40126 Bologna, Italy

来源：

CHROMATOGRAPHIA | 2002年 / 56卷 / 5-6期

关键词：

column liquid chromatography; pre-chromatographic derivatization; 4,7-phenanthroline-5,6-dione (phanquinone); amino acid drug determination;

D O I：

10.1007/BF02491934

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of 4,7-phenanthroline-5,6-dione (phanquinone) as a pre-chromatographic reagent for LC analysis of amino acids is proposed. The reagent reacts (30 min at 50degreesC) with primary amino group and the resulting adducts can be chromatographed under reversed-phase conditions (Phenyl-Hexyl Column). The experimental conditions for the derivatization and chromatographic separations are discussed. Application to the determination of several amino acids in pharmaceutical formulations is described.

引用

页码：289 / 294

页数：6