FenF:: Servicing the mycosubtilin synthetase assembly line in trans

被引：18

作者：

Aron, Zachary D. ^{[1
]}

Fortin, Pascal D. ^{[1
]}

Calderone, Christopher T. ^{[1
]}

Walsh, Christopher T. ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA

来源：

CHEMBIOCHEM | 2007年 / 8卷 / 06期

关键词：

biosynthesis; enzymes; kinetics; mycosubtilin; polyketides; transferases;

D O I：

10.1002/cbic.200600575

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

AT your service. We report the expression and characterization of FenF from mycosubtilin biosynthesis. This work represents the first kinetic and selectivity studies performed on an in trans AT domain servicing a polyketide synthase (PKS), and revealed a strong acyl-group specificity and broad promiscuity toward substrate carrier proteins. The lack of specificity in FenF-mediated malonyl transfer suggests that this protein might prove a powerful tool for combinatorial biosynthesis. (Chemical Equation Presented). © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：613 / 616

页数：4

共 16 条

[1] Characterization of a new tailoring domain in polyketide biogenesis: The amine transferase domain of MycA in the mycosubtilin gene cluster
Aron, ZD
Dorrestein, PC
Blackhall, JR
Kelleher, NL
Walsh, CT
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (43) : 14986 - 14987
[2] Convergence of isoprene and polyketide biosynthetic machinery:: Isoprenyl-S-carrier proteins in the pksX pathway of Bacillus subtilis
Calderone, Christopher T.
Kowtoniuk, Walter E.
Kelleher, Neil L.
Walsh, Christopher T.
Dorrestein, Pieter C.
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (24) : 8977 - 8982
[3] Structural and functional characterization of three polyketide synthase gene clusters in Bacillus amyloliquefaciens FZB 42
Chen, Xiao-Hua
Vater, Joachim
Piel, Joern
Franke, Peter
Scholz, Romy
Schneider, Kathrin
Koumoutsi, Alexandra
Hitzeroth, Gabriele
Grammel, Nicolas
Strittmatter, Axel W.
Gottschalk, Gerhard
Suessmuth, Roderich D.
Borriss, Rainer
[J]. JOURNAL OF BACTERIOLOGY, 2006, 188 (11) : 4024 - 4036
[4] Type I polyketide synthase requiring a discrete acyltransferase for polyketide biosynthesis
Cheng, YQ
Tang, GL
Shen, B
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (06) : 3149 - 3154
[5] CORNISHBOWDIN A, 1995, ANAL ENZYME KINETIC
[6] Malonyl-CoA:ACP transacylase from Streptomyces coelicolor has two alternative catalytically active nucleophiles
Dreier, J
Li, Q
Khosla, C
[J]. BIOCHEMISTRY, 2001, 40 (41) : 12407 - 12411
[7] The mycosubtilin synthetase of Bacillus subtilis ATCC6633:: A multifunctional hybrid between a peptide synthetase, an amino transferase, and a fatty acid synthase
Duitman, EH
Hamoen, LW
Rembold, M
Venema, G
Seitz, H
Saenger, W
Bernhard, F
Reinhardt, R
Schmidt, M
Ullrich, C
Stein, T
Leenders, F
Vater, J
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (23) : 13294 - 13299
[8] STUDIES ON MECHANISM OF FATTY ACID SYNTHESIS .26. PURIFICATION AND PROPERTIES OF MALONYL-COENZYME-A - ACYL CARRIER PROTEIN TRANSACYLASE OF ESCHERICHIA-COLI
JOSHI, VC
WAKIL, SJ
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1971, 143 (02) : 493 - +
[9] Enhancing the modularity of the modular polyketide synthases:: Transacylation in modular polyketide synthases catalyzed by malonyl-CoA:ACP transacylase
Kumar, P
Koppisch, AT
Cane, DE
Khoslaa, C
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (47) : 14307 - 14312
[10] ITURINS, A SPECIAL-CLASS OF PORE-FORMING LIPOPEPTIDES - BIOLOGICAL AND PHYSICOCHEMICAL PROPERTIES
MAGETDANA, R
PEYPOUX, F
[J]. TOXICOLOGY, 1994, 87 (1-3) : 151 - 174

← 1 2 →