Identification of macrophage migration inhibitory factor (MIF) in human skin and its immunohistochemical localization

被引：129

作者：

Shimizu, T

Ohkawara, A

Nishihira, J

Sakamoto, W

机构：

[1] HOKKAIDO UNIV,SCH MED,CANC RES INST,SAPPORO,HOKKAIDO 060,JAPAN

[2] HOKKAIDO UNIV,SCH MED,DEPT DERMATOL,SAPPORO,HOKKAIDO 060,JAPAN

[3] HOKKAIDO UNIV,SCH DENT,DEPT BIOCHEM,SAPPORO,HOKKAIDO 060,JAPAN

来源：

FEBS LETTERS | 1996年 / 381卷 / 03期

关键词：

human skin; immunohistochemistry; keratinocyte; macrophage migration inhibitory factor; reverse transcription-polymerase chain reaction;

D O I：

10.1016/0014-5793(96)00120-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The presence and tissue localization of macrophage migration inhibitory factor (MIF) in human skin were examined, Reverse transcription-polymerase chain reaction analysis revealed that MIF mRNA was expressed in both surgically obtained normal human epidermis and primary cultured human keratinocytes. The expression of MIF was further confirmed by Western blot analysis, which demonstrated a single band at about 12.5 kDa using a polyclonal antibody against human recombinant MIF. Immunohistochemical studies showed that MIF existed in human epidermis, especially in the basal layer, The pathophysiological role of MIF in human skin remains undefined; however, the present results indicate that MIF may play an important role in immunity, inflammation and cellular differentiation of epidermal cells.

引用

页码：199 / 202

页数：4

共 19 条

[1] KERATINOCYTES AS INITIATORS OF INFLAMMATION [J].