Inhibition of protein tyrosine phosphatases blocks calcium-induced activation of metaphase II-arrested oocytes of Xenopus laevis

被引：14

作者：

Bodart, JF

Béchard, D

Bertout, M

Rousseau, A

Gannon, J

Vilain, JP

Flament, S

机构：

[1] Univ Lille 1, Ctr Biol Cellulaire, Dev Biol Lab, UPRES EA 1033, F-59655 Villeneuve Dascq, France

[2] Imperial Canc Res Fund, Clare Hall Labs, S Mimms EN6 3LD, Herts, England

来源：

FEBS LETTERS | 1999年 / 457卷 / 02期

关键词：

oocyte; calcium; protein tyrosine phosphatase; activation; Xenopus;

D O I：

10.1016/S0014-5793(99)00986-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have studied the effect of a protein tyrosine phosphatases (PTP) inhibitor on calcium-induced activation of Xenopus laevis oocytes arrested at metaphase II. Ammonium molybdate microinjection blocked pronucleus formation following A23187 treatment while cortical granules still underwent exocytosis. Pronuclei still occurred in ammonium molybdate-injected oocytes following 6-DMAP addition. Changes that usually occurred following A23187 exposure were inhibited in the presence of ammonium molybdate in the oocyte: MAPK dephosphorylation, p34(cdc2) rephosphorylation and cyclin B2 and p39(mos) proteolysis. These results suggest that a PTP is involved in the activation of the ubiquitin-dependent degradation machinery. (C) 1999 Federation of European Biochemical Societies.

引用

页码：175 / 178

页数：4

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