Characterisation and determination of indole alkaloids in frog-skin secretions by electrospray ionisation ion trap mass spectrometry

被引：53

作者：

McClean, S ^{[1
]}

Robinson, RC ^{[1
]}

Shaw, C ^{[1
]}

Smyth, WF ^{[1
]}

机构：

[1] Univ Ulster, Sch Biomed Sci, Coleraine BT52 1SA, Londonderry, North Ireland

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2002年 / 16卷 / 05期

关键词：

D O I：

10.1002/rcm.583

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The characterisation of selected indole alkaloids in a quadrupole ion trap mass spectrometer is presented. Fragmentation profiles for tryptaraine, 5-hydroxytryptamine (5-HT), N'-methyl 5-hydroxytryptamine (N'-methyl 5-HT), N',N'-dimethyl 5-hydroxytryptamine (bufotenine), N',N',N'trimethyl 5-hydroxytryptamine (5-HTQ), and N',N'-dimethyl 5-metlioxytryptamine (5-MeODMT) are presented with proposed structures given for each product ion observed. Such MSn experiments can be used to differentiate the isobaric molecular ions of the compounds 5-HTQ (W) and 5-MeODMT (MH+). The quantitative determination of certain indole alkaloids in the skin secretions of the Australian Golden Bell frog, Litoria aurea, by LC/ESI-ion trap MS is also presented. The concentrations of 5-HT, N'-methyl 5-HT and 5-HTQ were found to be 2.68, 0.26 and 0.54 mug per mg of skin secretion, respectively. Copyright (C) 2002 John Wiley Sons, Ltd.

引用

页码：346 / 354

页数：9

共 26 条

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