Ghrelin stimulates insulin-induced glucose uptake in adipocytes

被引：60

作者：

Patel, AD ^{[1
]}

Stanley, SA ^{[1
]}

Murphy, KG ^{[1
]}

Frost, GS ^{[1
]}

Gardiner, JV ^{[1
]}

Kent, AS ^{[1
]}

White, NE ^{[1
]}

Ghatei, MA ^{[1
]}

Bloorn, SR ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Fac Med, Dept Metab Med, Nutr & Dietet Res Grp, London W12 0NN, England

来源：

REGULATORY PEPTIDES | 2006年 / 134卷 / 01期

基金：

英国医学研究理事会;

关键词：

GHS-R1(a); adipose tissue; des-acyl ghrelin;

D O I：

10.1016/j.regpep.2005.11.001

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The gastric and hypothalamic hormone ghrelin is the endogenous agonist of the growth hormone secretagogue receptor GHS-R1(a). Ghrelin stimulates growth hormone release and appetite via the hypothalamus. However, putative direct peripheral effects of ghrelin remain poorly understood. Rat adipose tissue expresses GHS-R1(a) mRNA, suggesting ghrelin may directly influence adipocyte function. We have investigated the effects of ghrelin oil insulin-stimulated glucose uptake in isolated white adipocytes in vitro. RT-PCR confirmed the expression of GHS-R1(a) mRNA in epididymal adipose tissue. However, GHS-R1(a) expression was not detected in the peri-renal fat pads. Ghrelin increased insulin-stimulated deoxyglucose uptake in isolated white adipocytes extracted from the epididymal fat pads of male Wistar rats. Ghrelin 1000 nM significantly increased deoxyglucose uptake by 55% in the presence of 0.1 nM insulin. However, ghrelin administration in the absence of insulin had no effect on adipocyte deoxyglucose uptake, suggesting that ghrelin acts synergistically with insulin. Des-acyl ghrelin, a major circulating non-octanylated form of ghrelin, had no effect oil insulin-stimulated glucose uptake. Furthermore, acylated ghrelin had no effect oil deoxyglucose uptake in adipocytes from peri-renal fat pads Suggesting that ghrelin may influence glucose uptake via the GHS-R1(a). Ghrelin therefore appears to directly potentiate adipocyte insulin-stimulated glucose uptake in selective adipocyte Populations. Ghrelin may play a role in adipocyte regulation Of glucose homeostasis. (C) 2005 Elsevier B.V. All rights reserved.

引用

页码：17 / 22

页数：6

共 36 条

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