Killing of Bacillus subtilis spores by a modified Fenton reagent containing CuCl2 and ascorbic acid

被引：18

作者：

Shapiro, MP ^{[1
]}

Setlow, B ^{[1
]}

Setlow, P ^{[1
]}

机构：

[1] Univ Connecticut, Dept Biochem, Ctr Hlth, Farmington, CT 06032 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2004年 / 70卷 / 04期

关键词：

D O I：

10.1128/AEM.70.4.2535-2539.2004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bacillus subtilis spores were killed by CUCl2-ascorbic acid, chloride ions were essential for killing of spores, and spores with defective coats were killed more rapidly. CUCl2-ascorbic acid did not damage spore DNA, and spores killed by this reagent initiated germination. However, spores killed by CuCl2-ascorbic acid may have damage to their inner membrane..

引用

页码：2535 / 2539

页数：5

共 37 条

[1]

[Anonymous], 1990, Molecular biological methods for Bacillus, DOI DOI 10.1111/J.1751-1097.1991.TB02087.X

[2] Characterization of yhcN, a new forespore-specific gene of Bacillus subtilis [J].

Bagyan, I ;

Noback, M ;

Bron, S ;

Paidhungat, M ;

Setlow, P .

GENE, 1998, 212 (02) :179-188

[3] Killing of Bacillus spores by aqueous dissolved oxygen, ascorbic acid, and copper ions [J].

Cross, JB ;

Currier, RP ;

Torraco, DJ ;

Vanderberg, LA ;

Wagner, GL ;

Gladen, PD .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (04) :2245-2252

[4]

Driks A, 1999, MICROBIOL MOL BIOL R, V63, P1

[5] PREVENTION OF DNA DAMAGE IN SPORES AND INVITRO BY SMALL, ACID-SOLUBLE PROTEINS FROM BACILLUS SPECIES [J].

FAIRHEAD, H ;

SETLOW, B ;

SETLOW, P .

JOURNAL OF BACTERIOLOGY, 1993, 175 (05) :1367-1374

[6] Killing of spores of Bacillus subtilis by peroxynitrite appears to be caused by membrane damage [J].

Genest, PC ;

Setlow, B ;

Melly, E ;

Setlow, P .

MICROBIOLOGY-SGM, 2002, 148 :307-314

[7]

GERHARDT P, 1972, SPORES, V5, P68

[8]

HILL PJ, 1994, SOC APPL BACTERIOL S, V76, pS129

[9] DNA DAMAGE AND OXYGEN RADICAL TOXICITY [J].

IMLAY, JA ;

LINN, S .

SCIENCE, 1988, 240 (4857) :1302-1309

[10] Superoxide accelerates DNA damage by elevating free-iron levels [J].

Keyer, K ;

Imlay, JA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (24) :13635-13640

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