Over-expression of an S-adenosylmethionine-dependent methyltransferase leads to an extended lifespan of Podospora anserina without impairments in vital functions

被引：45

作者：

Kunstmann, Birgit ^{[1
]}

Osiewacz, Heinz D. ^{[1
]}

机构：

[1] Goethe Univ Frankfurt, Dept Biol Sci & Cluster Excellence Macromol Compl, Inst Mol Biosci, D-60438 Frankfurt, Germany

来源：

AGING CELL | 2008年 / 7卷 / 05期

关键词：

flavonoids; mitochondria; o-methyltransferase; oxidative stress; Podospora anserina; reactive oxygen species;

D O I：

10.1111/j.1474-9726.2008.00412.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

PaMTH1, a putative methyltransferase previously described to increase in abundance in total protein extracts during aging of Podospora anserina is demonstrated to accumulate in the mitochondrial cell fraction of senescent cultures. The protein is localized in the mitochondrial matrix and displays a methyltransferase activity utilizing flavonoids as substrates. Constitutive over-expression of PaMth1 in P. anserina results in a reduced carbonylation of proteins and an extended lifespan without impairing vital functions suggesting a protecting role of PaMTH1 against oxidative stress.

引用

页码：651 / 662

页数：12

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