Protein kinase B/AKT isoform 2 drives migration of human mesenchymal stem cells

被引：31

作者：

Bulj, Zrinka ^{[2
,6
]}

Duchi, Serena ^{[1
]}

Bevilacqua, Alessandro ^{[3
,4
]}

Gherardi, Alessandro ^{[3
,4
]}

Dozza, Barbara ^{[1
,5
]}

Piccinini, Filippo ^{[3
,4
]}

Mariani, Giulia Adalgisa ^{[2
]}

Lucarelli, Enrico ^{[1
]}

Giannini, Sandro ^{[1
,5
]}

Donati, Davide ^{[1
,5
]}

Marmiroli, Sandra ^{[6
]}

机构：

[1] Rizzoli Orthopaed Inst, Osteoarticular Regenerat Lab, I-40136 Bologna, Italy

[2] Dept Biomed Sci, Cellular Signalling Lab, I-40126 Bologna, Italy

[3] Adv Res Ctr Elect Syst Informat & Commun Technol, I-40125 Bologna, Italy

[4] Dept Elect Comp Sci & Syst DEIS, I-40136 Bologna, Italy

[5] Dept Biomed Sci, I-40126 Bologna, Italy

[6] Dept Surg Med Dent & Morphol Sci, Cellular Signalling Lab, I-41124 Modena, Italy

来源：

INTERNATIONAL JOURNAL OF ONCOLOGY | 2013年 / 42卷 / 01期

关键词：

mesenchymal stem cells; phosphoinositide-3 kinase/protein kinase B pathway; cell migration; protein kinase B Inhibitors; wound healing; DISTINCT ROLES; BONE-MARROW; CANCER; AKT; SUBSTRATE; DIFFERENTIATION; PALLADIN; MOTILITY; INVASION;

D O I：

10.3892/ijo.2012.1700

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

This study was designed to investigate the migratory behavior of adult human mesenchymal stem cells (MSC) and the underlying mechanism. Cell migration was assessed by transwell, wound healing and time-lapse in vivo motility assays. Pharmacological inhibitors were used to determine the potential mechanism responsible for cell migration and invasion. The tests that were implemented revealed that MSC were fairly migratory. Protein kinase B (AKT) was strongly activated at the basal level. Through our analyses we demonstrated that pharmacological inactivation of AKT2 but not AKT1 significantly decreased cell migration and invasion. Although preliminary, collectively our results indicate that AKT2 activation plays a critical role in enabling MSC migration.

引用

页码：118 / 126

页数：9

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